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Understanding effects of nutrient availability on algal-fungal mutualism

Background: Microbial interactions are not static but instead occur on a continuum from cooperation to competition, affected by the prevailing environmental conditions. Among these, nutrient availability is particularly important, as heterotrophic microorganisms can benefit from algal photosynthates while, at the same time, competing with the alga for essential nutrients such as nitrogen. Nutrient supply can therefore exert a strong influence on the outcome of an interaction.

Project Description: This project aims to investigate whether and how nutrient availability changes the outcome of the interaction between the alga Chlamydomonas reinhardtii and the soil-borne filamentous fungus Verticillium dahliae (Prockl et al., 2026). By systematically modifying growth medium compositionsincluding nitrogen and phosphorus concentrations, we will assess whether varying culturing conditions change the interaction outcome between the two partners. When shifts in interaction outcomes are observed, we will further explore potential mechanisms driving these changes.

Technologies and Methods: 

  • Microscopy 

  • Algal–fungal co-culturing and axenic cultivation 

  • Spectrophotometric growth assays and cell counting

  • Biomass quantification and gene expression analysis by real-time qPCR

  • Statistical analysis of interaction outcome data

  • Method development (depending on project progress)

 

Supervisor: Zoe Prockl

Relevant literature:

Prockl Z, Neubacher U, Rovenich H. 2026The model alga Chlamydomonas reinhardtii forms mutualistic interactions with Verticillium fungi. bioRxiv doi:10.64898/2026.05.20.726472.